High-power, dual-wavelength laser for multiphoton microscopy by Coherent

The Chameleon Discovery dual-wavelength laser from Coherent combines continuous tunability from 680 to 1300 nm, 1.5 W of output power (at 1040 nm), and 100 fs pulsewidth for multiphoton microscopy applications.

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The Chameleon Discovery dual-wavelength laser from Coherent (Santa Clara, CA) combines continuous tunability from 680 to 1300 nm, 1.5 W of output power (at 1040 nm), and 100 fs pulsewidth for multiphoton microscopy applications. Excitation of all popular fluorophores, including eGFP, YFP, and all the red-shifted proteins, is possible. Secondary output at 1040 nm provides high power, short pulses (160 fs), and optimum beam parameters for simultaneous, multiwavelength excitation of multiple probes and/or photoactivation of opsins for optogenetics applications. These applications include optogenetic experiments based on established schemes using longer wavelength opsins like C1V1, as well as alternative wavelength pairings based on red-shifted calcium indicators of the GCaMP family currently under development. Additional applications include techniques such as coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS).

For more information, please visit www.coherent.com.


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