Semrock has augmented its flagship BrightLine family of fluorescence filters with a series of high performance fluorescence sets that are specially designed and optimized for laser excitation.
The filter sets are crafted for the most popular lasers used in fluorescence imaging, including the newer all-solid-state lasers that are rapidly replacing older gas-laser technology. The new excitation filters promise excellent transmission at the designated laser wavelengths, combined with very low ripple passbands. According to the company, the transition from reflection to transmission for both the excitation and emission filters is exceptionally steep (less than 2.5% of the longest laser wavelength) to enable maximum capture of even weak signals close to the laser line. And the matched dichroics reportedly exhibit excellent laser reflection for all polarizations, and transition rapidly to an exceptionally wide and low-ripple fluorescence passband, with the flatness and surface cosmetic properties needed for excellent imaging performance.
BrightLine Laser Fluorescence filter sets are designed for use in laser-scanning and spinning-disk confocal microscopes, as well as total-internal-reflection fluorescence (TIRF) microscopes. They are also appropriate for any fluorescence microscope or imaging system that demands premium spectral performance and/or beamsplitter flatness, such as structured illumination systems for reduction of out-of-plane noise.
Like all of Semrock's optical filters, the laser fluorescence filter sets are exclusively manufactured with hard-coated Ion-Beam Sputtering technology for Semrock's "no burn-out" reliability, and they are backed by a five year warranty. The filter sets ship quickly from stock.