Researchers at the Massachusetts Institute of Technology (MIT; Cambridge, MA) have shown that an optogenetic approach involving LEDs that flicker at a specific frequency can substantially reduce the beta amyloid plaques seen in Alzheimer's disease. The method was shown in the visual cortex of mice.
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This treatment appears to work by inducing brain waves known as gamma oscillations, which the researchers discovered help the brain suppress beta amyloid production and invigorate cells responsible for destroying the plaques.
Alzheimer's disease is characterized by beta amyloid plaques that are suspected to be harmful to brain cells and to interfere with normal brain function. Previous studies have hinted that Alzheimer's patients also have impaired gamma oscillations. These brain waves, which range from 25 to 80 Hz (cycles per second), are believed to contribute to normal brain functions such as attention, perception, and memory.
In a study of mice that were genetically programmed to develop Alzheimer's but did not yet show any plaque accumulation or behavioral symptoms, Li-Huei Tsai, the Picower Professor of Neuroscience, director of MIT's Picower Institute for Learning and Memory, and senior author of the study, and her colleagues found impaired gamma oscillations during patterns of activity that are essential for learning and memory while running a maze.
|"…[I]f humans behave similarly to mice in response to this treatment, I would say the potential is just enormous, because it's so noninvasive, and it's so accessible," says Li-Huei Tsai, the Picower Professor of Neuroscience, when describing a new treatment for Alzheimer's disease. (Photo: Bryce Vickmark)|
Next, the research team stimulated gamma oscillations at 40 Hz in a brain region called the hippocampus, which is critical in memory formation and retrieval. These initial studies relied optogenetics, which allows scientists to control the activity of genetically modified neurons by shining light on them. Using this approach, the researchers stimulated certain brain cells known as interneurons, which then synchronize the gamma activity of excitatory neurons. After an hour of stimulation at 40 Hz, the researchers found a 40-50% reduction in the levels of beta amyloid proteins in the hippocampus. Stimulation at other frequencies, ranging from 20 to 80 Hz, did not produce this decline.
Tsai and colleagues then began to wonder if less-invasive techniques might achieve the same effect. Tsai and Emery Brown, the Edward Hood Taplin Professor of Medical Engineering and Computational Neuroscience, a member of the Picower Institute, and an author of the paper, came up with the idea of using an external stimulus (light) to drive gamma oscillations in the brain. The researchers built a simple device consisting of a strip of LEDs that can be programmed to flicker at different frequencies.
Using this device, the researchers found that an hour of exposure to light flickering at 40 Hz enhanced gamma oscillations and reduced beta amyloid levels by half in the visual cortex of mice in the very early stages of Alzheimer's. However, the proteins returned to their original levels within 24 hours.
The researchers then investigated whether a longer course of treatment could reduce amyloid plaques in mice with more advanced accumulation of amyloid plaques. After treating the mice for an hour a day for seven days, both plaques and free-floating amyloid were markedly reduced. The researchers are now trying to determine how long these effects last.
Furthermore, the researchers found that gamma rhythms also reduced another hallmark of Alzheimer's disease: the abnormally modified Tau protein, which can form tangles in the brain.
Tsai's lab is now studying whether light can drive gamma oscillations in brain regions beyond the visual cortex, and preliminary data suggest that this is possible. They are also investigating whether the reduction in amyloid plaques has any effects on the behavioral symptoms of their Alzheimer's mouse models, and whether this technique could affect other neurological disorders that involve impaired gamma oscillations.
The researchers also performed studies to try to figure out how gamma oscillations exert their effects. They found that after gamma stimulation, the process for beta amyloid generation is less active. Gamma oscillations also improved the brain's ability to clear out beta amyloid proteins, which is normally the job of immune cells known as microglia. In Alzheimer's patients, microglia cells become very inflammatory and secrete toxic chemicals that make other brain cells more sick. However, when gamma oscillations were boosted in mice, their microglia underwent morphological changes and became more active in clearing away the beta amyloid proteins.
The researchers also sequenced messenger RNA from the brains of the treated mice and found that hundreds of genes were over- or underexpressed, and they are now investigating the possible impact of those variations on Alzheimer's disease.
Further research will be needed to determine if a similar approach could help Alzheimer's patients, Tsai says.
Full details of the work appear in the journal Nature; for more information, please visit http://dx.doi.org/10.1038/nature20587.