Cell Imaging/Cell Analysis: EUV-enabled spectrometry images cells in 3D at nanoscale

Researchers at Colorado State University (Fort Collins, CO) have developed a spectral imaging instrument that maps cellular composition in 3D at the nanoscale.

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Researchers at Colorado State University (Fort Collins, CO) have developed a spectral imaging instrument that maps cellular composition in 3D at the nanoscale.1 The system allows study of cells at approximately 100% greater detail than previously possible, enabling observation of cell response to drugs.

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Precise positioning of cell samples allows laser drilling, which in turn enables chemical characterization and nanoscale anatomical charting in 3D.

The researchers explain that earlier laser-based mass-spectral imaging could identify the chemical composition of a cell and could map its surface in 2D at the microscale, but could not chart cellular anatomy in nanoscale or in 3D.

The instrument features a laser able to produce a hot, dense plasma stream that acts as a gain medium for generating extreme ultraviolet (EUV) pulses. When properly focused, the laser drills a tiny hole into a cell sample, enabling ions to evaporate from the cell surface. These ions can be separated and identified to determine chemical composition, and used to chart the anatomy of a cell.

1. I. Kuznetsov, NatureCommun., 6, 6944, doi:10.1038/ncomms7944 (2015).

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