Super-sensitive molecular imaging differentiates toxins using carbon nanotubes

CAMBRIDGE, MA, USA--MIT researchers report the first demonstration that nanoscale sensors can be used to detect and image multiple types of molecules in cells at the same time--and with far greater sensitivity than that of fluorescent dyes. The researchers used the sensors to detect various DNA-damaging substances, including cancer drugs and toxins.

CAMBRIDGE, MA, USA--Researchers at MIT have found that carbon nanotubes can serve as highly sensitive biological sensors for detecting single molecules in living cells in real time, according to MIT's Technology Review. Their study, published online in Nature Nanotechnology, is the first demonstration that nanoscale sensors can be used to detect and image multiple types of molecules in cells at the same time, at a sensitivity that far exceeds that of fluorescent dyes, the standard tool for molecular imaging.

The researchers used the sensors to detect substances that damage DNA, including certain cancer drugs and toxins. They hope sensors will eventually be used to monitor the effectiveness of chemotherapy drugs, track molecular interactions in cells, and test for low levels of toxins in the environment.

The structures fluoresce when exposed to near-infrared light. This property is useful for biological imaging because near-infrared light can penetrate tissues more deeply than visible light can. And because cells do not fluoresce when exposed to near-infrared light, an near-infrared light-emitting sensor is easier to spot.

The sensors, developed in chemical engineering professor Michael Strano's lab, are single-wall carbon nanotubes wrapped with a small piece of DNA. When a target molecule binds to the DNA, it causes a change in the light emitted by the nanotube; the change in the light signal can be detected by a microscope. The researchers used the sensors to detect molecules that damage DNA, including chemotherapy drugs, free radicals, and hydrogen peroxide.

Strano says that the sensors offer several important advantages over fluorescent dyes. Not only can they detect and locate molecules, but different types of molecules will affect the properties of the emitted light differently. "When a molecule binds to it, it can change the wavelength or intensity of light that comes out," Strano says. "Every toxin has a unique signature. So you're not just detecting it; you can say something about what kind of toxin it is or what kind of drug it is."

In this study, the researchers used two different types of carbon nanotubes to distinguish between four different classes of toxins in living cells, but Strano believes that the sensors could be configured to detect many molecules within a sample or cell at once.

Furthermore, the nanotube sensors can detect very small molecules that would be difficult to identify with other technologies, and at very low concentrations.

More information:
Read the article in MIT's Technology Review.

Posted by Barbara G. Goode, barbarag@pennwell.com.

More in Biomedicine