AnaSpec (San Jose, CA) recently expanded their green fluorescent protein (GFP) antibodies offerings to include two monoclonals raised in mice and a polyclonal raised in chicken. Purified GFP was used as antigen. Antibody reactivity was confirmed by an enzyme-linked immunosorbent assay (ELISA) and specificity was confirmed by a Western blot using GFP protein. These antibodies also recognize other GFP mutant forms.
Green fluorescent protein (GFP) is a 27-kDa protein originally cloned from jellyfish. This exceptional protein absorbs blue light up to 395 nm and emits green light up to 509 nm without any requirement for exogenous substrates and cofactors.1 These unique intrinsic fluorescence properties make GFP an invaluable tool in cell biology research in applications such as monitoring gene expression and protein localization of GFP-tagged proteins in-vivo. Other applications of GFP include assessment of protein-protein interactions through the yeast two-hybrid system and measurement of distance between proteins through fluorescence resonance energy transfer (FRET) protocols. Several mutant forms of GFP have been developed that fluoresce more intensely and have shifted excitation maximum when compared to the wild type GFP, making them useful for fluorescence-activated cell sorting (FACS), fluorescence microscopy and double-labeling applications.2,3
1. B. Cormack et al., Gene 173: 33 (1996)
2. R. Rizzuto et al. Curr. Biol. 6: 183 (1996)
3. M. Chalfie et al., Science 263: 802 (1994)
Posted by Lee Mather
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