Thorlabs licenses random-access multiphoton microscopy technology

Thorlabs will help to commercialize the two-photon random access mesoscope developed at HHMI's Janelia Research Campus.

Thorlabs (Newton, NJ) has entered into a licensing agreement with the Howard Hughes Medical Institute (HHMI; Chevy Chase, MD) to commercialize the two-photon random access mesoscope (2p-RAM) developed in Karel Svoboda's lab at HHMI's Janelia Research Campus (Ashburn, VA) for in vivo imaging of large brain volumes (up to Ø5 × 1 mm) with single neuron resolution.

Related: Two-photon microscopy inventors win Brain Prize

Even for the simplest animal behaviors, there are a large number of neurons operating in concert over multiple areas of the brain. With currently available high-resolution, two-photon microscopes, it is possible to image the activity of individual neurons in awake, behaving mammals but the field of view is small (<1 mm), limiting our understanding of neural activity within multiple regions of the brain. With large-field-of-view microscopes, it is possible to image the entire brain, but individual neurons cannot be resolved, particularly in the axial direction.

The ongoing research efforts in the Svoboda lab have been aimed at overcoming the hardware and software challenges that currently limit our understanding of neural circuits through the development of a mesoscale two-photon microscope capable of subcellular resolution over a field of view that spans multiple brain areas.

Thorlabs expects to have the 2p-RAM mesoscope available for sale starting in Q1 2017. The microscope will also be incorporated into one of its demonstrations at the upcoming 2016 Meeting of the Society for Neuroscience, taking place November 12–16th in San Diego, CA.

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