*Depth of field? Resolution? Traditionally, using OCT meant choosing between these two critical parameters. But interferometric synthetic aperture microscopy (ISAM), an advance in computed imaging, lets imaging pros have both, simultaneously. Its first application: breast cancer imaging.*

*By***P. Scott Carney and Stephen A. Boppart**

Optical coherence tomography (OCT), a combination of broadband interferometry and scanning-light microscopy, provides axial depth ranging and enables two- and three-dimensional imaging by combining data acquired sequentially in space.^{1} Many OCT variants use light sources with various levels of coherence; all can acquire data interferometrically across a broad spectral range.

Because the axial resolution is proportional to the inverse of the source-detector bandwidth, it is possible to improve resolution by boosting source bandwidth. While the transverse resolution is inversely proportional to the numerical aperture (NA) of the optical focusing system, the images generated are usable only within the confocal region—that is, within a Rayleigh range of the focal plane. Unfortunately, as the NA increases, this confocal region diminishes—and so at some point practitioners must choose between depth of focus (the confocal length) and transverse resolution. But a new computed imaging technique, interferometric synthetic aperture microscopy (ISAM), makes such a compromise no longer necessary.

An advance in computed imaging requires developments on three fronts: hardware, theory, and available computing power. This is why, although the means to make measurements—the essential theory and the key numerical tool, the fast Fourier transform (FFT)—had been long established, it wasn't until computational power became available in the early 1970s that the revolutionary advances of computed tomography (CT) and magnetic resonance imaging (MRI) became possible.

In optics, the unambiguous determination of object structure without strong prior assumptions requires measurements from which the electric field (both phase and amplitude) may be inferred. The methods of holography, for instance, were developed precisely for the purposes of post-processing.^{2} That is, holography was proposed as a way of recording and later manipulating the field to obtain standard microscopic images. It was later observed that the hologram, or rather a series of holograms, may be seen to encode the underlying object structure and that the structure may be computed.^{3} For a given plane-wave illumination, the plane-wave amplitude of the scattered field is proportional to a particular element of the Fourier transform of the sample susceptibility. Diffraction tomography, as this technique came to be known, requires the acquisition of many phase-coherent holograms—a difficult accomplishment. But some good progress has been made over the years and is discussed in the literature.^{4}

Gabor's original idea to reprocess the field to produce a better image has seen a resurgence in the digital age. In holographic refocusing methods, the digital hologram is convolved with a complex transfer function in place of a physical lens. This is accomplished in a computationally efficient way by taking a FFT, multiplying by the appropriate function, and taking an inverse FFT (iFFT). In essence, OCT provides a multifrequency hologram and the refocusing method may be applied frequency-by-frequency to effectively move the focal plane even to planes outside the Rayleigh range.^{5} However, the image in the plane of the original focus is then out of focus.

The key idea in diffraction tomography is to forego processing the field to obtain an intensity that better resembles the object in favor of a direct solution of the inverse problem to determine the underlying object structure. A single, 2-D hologram made with monochromatic fields clearly cannot fully encode an arbitrary 3-D function; hence the need for multiple holographic views in diffraction tomography. However, OCT provides multiple holograms at different frequencies from a single 2-D scan, or from a single full-field image. These data are not generally enough to solve the inverse problem either since an arbitrary object is necessarily a function of three spatial coordinates and frequency. However, OCT itself is only able to provide images of structure under the assumption that the susceptibility may be written as a simple product of a spatially dependent function and a frequency-dependent function; that is, it is separable. In that case, the inverse problem may be solved and, in principle, object structure can be reconstructed everywhere simultaneously.

FIGURE 1. The width of the point-spread functions for interferometric synthetic aperture microscopy (ISAM) and optical coherence tomography (OCT) are shown as a function of distance from focus. (Image courtesy University of Illinois at Urbana–Champaign) |

**ISAM**

A new method in computed optical imaging is thus timely. The computing power available in the modern desktop computer provides the means to process large data sets with the complexity needed to implement FFTs and interpolate data; various OCT instruments provide phase-coherent, co-registered holograms across multiple frequencies with ever-increasing speed of acquisition, and the theory is now in place to take advantage of these developments. Our team at the University of Illinois at Urbana–Champaign has solved the inverse problem for several OCT variants: planar scanning, rotational scanning, full-field, and partially coherent full-field OCT.^{6} We refer to these modalities collectively as interferometric synthetic aperture microscopy (ISAM), a name alluding to its connection to other synthetic-aperture techniques.^{7}

The inverse problem may be cast in a form amenable to fast implementation. In two separate asymptotic regimes, the data may be simply related to the object structure by the equation *Š(Q,k)*=*K(Q,k)ñ*(*Q*,−√4*k*^{2}−*Q*^{2}).^{8} Here *Š* denotes the 2-D Fourier transform of the data with respect to the scanning coordinates, *ñ* is the 3-D Fourier transform of the susceptibility of the object (the thing we seek), *k* is the wavenumber, and *K* is a known complex prefactor. The simultaneous reconstruction of the object everywhere is accomplished by resampling the data onto a new grid with coordinate *ß* such that k=√*ß*^{2}+*Q*^{2}/2 so as to obtain the Fourier transform of the susceptibility function on a uniform Cartesian grid. The 3-D object is then reconstructed by dividing by the appropriately regularized value of the prefactor *K* and taking the inverse Fourier transform. With noise-free data, the reconstruction is spatially uniform with the resolution everywhere equal to the resolution achieved in the focal plane of the system. In the presence of noise, the signal-to-noise ratio (SNR) falls off as the inverse of the distance from focus at distances larger than the Rayleigh length, but the point spread function remains unchanged as was recently verified.^{9} The average point-spread function with a phantom of sub-resolution-sized particles for OCT and ISAM can be measured (see Fig. 1). The oscillations seen in the OCT data result at large distance from the focus are not due to noise but instead arise because adjacent scatterers increasingly produce signals that interfere and form high-spatial-frequency structure, a phenomenon sometimes called speckle. The measurements were made using an achromatic doublet of focal length 12 mm, producing a theoretical focal-plane resolution of 4.4 μm (full-width half-maximum or FWHM) and a (free-space) Rayleigh range of 52 μm.

FIGURE 2. An OCT image of rat adipose tissue (a) is compared to an ISAM reconstruction from the same data (b); the images are nearly identical. The field of view is 500 × 500 μm. (Images courtesy University of Illinois at Urbana–Champaign) |

An OCT image of rat adipose tissue can be obtained 53 μm below the surface and 390 μm above focus and compared to an ISAM reconstruction from the same data (see Fig. 2). The experiment is the same as described in a recent paper from Ralston and colleagues, but the depths are different.10 While the OCT image is badly defocused, the ISAM image clearly shows the adipose tissue microstructure.

**Small but mighty**

Among other things, ISAM enables the development of a small-form-factor instrument that has a fixed focal plane and high resolution, but large depth-of-field limited only by SNR. Such an instrument could greatly expand the reach of low-coherence optical imaging in medicine. We are commercializing this technology through a company we cofounded called Diagnostic Photonics (www.diagnosticphotonics.com). Our initial target is in breast-conserving surgery or lumpectomy procedures. Here, the current standard of care includes post-operative histology for inspection of the margins of excised tissue. With ISAM, we plan to offer an in situ view of high-resolution cell and tissue microstructure over larger volumes in order to reduce re-operation rates. We expect other applications to follow. «

**ACKNOWLEDGMENTS**

The authors would like to thank Dr. Steven Adie for help in preparing images, as well as acknowledge that a grant from the National Institutes of Health (NIH)/National Cancer Institute (NCI), R01 EB012479, partly supported their ISAM research.

**REFERENCES**

1. D. Huang et al., *Science*, 254, 1178-1181 (1991)

2. D. Gabor, "Microscopy by recorded wavefronts," *Proc. Royal Soc. London*, 197, 454-487 (1949)

3. E. Wolf, *Optical Comm.*, 1, 153-156 (1969)

4. O. Haeberlé et al., *J. Mod. Opt.*, 57, 686-699 (2010)

5. J.P. Rolland et al., *Proc. SPIE*, 7372, 73721K (2009)

6. T.S. Ralston et al., *J. OSA*, A23, 1027-1037 (2006)

7. B.J. Davis et al., *Sensors*, 8, 3903-3931 (2008)

8. B.J. Davis et al., *J. OSA*, A24, 2527-2542 (2007)

9. T.S. Ralston et al., *Opt. Lett.*, 35, 1683-1685 (2010)

10. T.S. Ralston et al., *Nature Phys.*, 3, 129-134 (2007)

**P. SCOTT CARNEY*** is an associate professor and ***STEPHEN A. BOPPART*** is a professor in the Department of Electrical and Computer Engineering at the Beckman Institute for Advanced Science and Technology at the University of Illinois Urbana–Champaign, 405 North Mathews Avenue, Urbana, IL 61801; e-mail: carney@illinois.edu; http://illinois.edu.*

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