We have invited three renowned researchers, Prof. E. O. Potma to introduce parts of his research and the latest developments of the CARS technique, Jacco van Rheenen, PhD, who gives an overview of deep imaging of living cells in the infrared range with OPO and Dr. Martin Anger who shows some outstanding applications done with High Content Screening Automation.

Presentation 1:

OPO - Deep imaging with extended wavelength range up to 1300 nm
Intravital imaging by multiphoton microscopy provides a detailed picture of cellular interactions and subcellular structures deep within tissues. Optical parametric oscillators (OPO) are used for multiphoton excitation with wavelengths between 1100 and 1300 nm. This gives access to the full range of available fluorophores including novel red fluorescent proteins for intravital microscopy. The OPO can be used for simultaneous multicolor imaging as well as multimodal approaches.

Presentation 2:

Biological Applications with Coherent Anti-Stokes Raman Spectroscopy - CARS
Combining confocal microscopy with Coherent anti-Stokes Raman Scattering (CARS) microscopy overcomes the limitations of dye-based imaging methods. CARS microscopy is a non-toxic, non-destructive and minimally invasive technology which images the characteristic intrinsic vibrational contrast of the molecules in the sample.. In consequence the specimen (living cells, tissue, whole organs and even living animals) remains almost unaffected and can be analyzed in their native state.

Presentation 3:

Leica HCS A
Leica HCS A - High Content Screening Automation for the first time offers high content screening on research microscopes by the combination of high-resolution true confocal point scanning technology or integrated widefield systems with the LAS AF MATRIX M3 software. The automation allows to run more experiments in less time and to obtain more research information and statistically relevant data. The new solution provides great flexibility for individual experiments and unbiased image acquisition. The new HCS-A mark and find application allows experiment on multiple, individual positions taught in. Especially for moving objects, intelligent single object tracking will keep mark and find objects within the middle of the image.