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Total Internal Reflection Fluorescence

Total Internal Reflection Fluorescence news and technical articles from BioOptics World. Search Total Internal Reflection Fluorescence latest and archived news and articles

  1. Optics/Fluorescence Imaging: Facilitating fluorescence with optical filtering

    Magazine Articles

    Mon, 11 Jul 2016

    laser excitation, such as confocal and total internal reflection fluorescence imaging, the most appropriate laser wavelengths ..... emission bands. Controlling RWD for TIRF Total internal reflection fluorescence (TIRF) microscopy was developed to restrict

  2. Fluorescence Imaging: Optical filtering basics for life sciences

    Magazine Articles

    Mon, 14 Dec 2015

    could be detrimental to any imaging system. In life sciences, this is critical for applications such as total internal reflection fluorescence (TIRF) and structured illumination microscopy. FIGURE 4. Dichroic beamsplitter measured at 45°. Multiband

  1. Zeiss partners with Molecular Imaging Platform at McGill University Health Centre

    Online Articles

    Fri, 11 Dec 2015

    CSU-X1 and two EMCCD cameras for high-sensitivity, dual-channel imaging. The system also offers total internal reflection fluorescence (TIRF) microscopy and fluorescence recovery after bleaching (FRAP) with live cell specimens. Additional

  2. MICROSCOPY/OPTOGENETICS: Neuroscience 2014 emphasizes instrumentation

    Magazine Articles

    Tue, 20 Jan 2015

    easy-to-use operation with two colors and no special dyes; the company says that objectives make total internal reflection fluorescence (TIRF) or structured illumination hardware unnecessary. The company hosted speakers from the Stanford

  3. OPTICAL MICROSCOPY/OPTOGENETICS: Simultaneous, multi-color scanning with diode lasers

    Magazine Articles

    Thu, 1 Nov 2012

    different depths are color-coded. (Image courtesy of Lars Hufnagel, EMBL, Heidelberg, Germany) Total internal reflection fluorescence (TIRF) microscopy is well suited for the analysis of cellular processes near the plasma membrane. Specialized

  4. BIOPHOTONICS RESEARCH FUNDING: Flexible funding options for biophotonics

    Magazine Articles

    Tue, 19 Nov 2013

    eight-instrument imaging core facility was ready for use. That core included fluorescence, confocal, total internal reflection fluorescence (TIRF), two-photon, and transmission electron microscopes. Over the next two years, the core facility

  5. Toptica Photonics multi-laser engine for multi-color microscopy and flow cytometry

    Online Articles

    Tue, 14 Dec 2010

    reflective surfaces Typical applications include: Flow cytometry Confocal microscopy Live-cell imaging Total Internal Reflection Fluorescence (TIRF) Fluorescence Recovery After Photobleaching (FRAP) High throughput screening Genome analyzers

  6. Microscopy uncovers lipid that drives cell polarity development

    Online Articles

    Mon, 20 Feb 2012

    Das. Then, Das created small bits of manmade, reconstituted membrane to test the mechanism. Using total internal reflection fluorescence ( TIRF ) microscopy, the team was able to show that the charge of the membrane does indeed determine

  7. Photometrics' "intelligent" camera aims to revolutionize viewing, evaluation of scientific data

    Online Articles

    Tue, 10 Feb 2009

    PALM), stochastic optical reconstruction microscopy (STORM), spinning disk confocal microscopy, total internal reflection fluorescence (TIRF) microscopy, cell trafficking studies, live-cell fluorescent protein imaging, and single

  8. MICROSCOPY/CELL BIOLOGY: TIRF and confocal microscopy illustrate wound healing dynamics

    Magazine Articles

    Thu, 1 Mar 2012

    functions as wound healing and cancer metastasis, which require that many cells move together. Now, total internal reflection fluorescence (TIRF) microscopy is helping to understand how endocytosisthe process by which cells take in substances

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