LIGHT MICROSCOPY/CELL BIOLOGY: $250 DIY light microscope tracks cell motility

Light microscopy systems for measuring cell motility can cost hundreds of thousands of dollars. But a PhD student at Brunel University London's College of Health and Life Sciences (Uxbridge, England) built his own inverted microscope for an estimated $250 by adapting three low-cost USB microscopes he purchased online.

The finished Low-Cost Motility Tracking System (LOCOMOTIS) adapted by Adam Lynch to study snail immune systems.

In learning about cell motility tracking, Adam Lynch, a student in the College's Institute for the Environment, learned how a snail's immune system responds to chemical pollutants present in water, which might influence transmission of Schistosome parasites to humans (the parasites can induce chronic infection of the urinary tract or intestines in humans). Lynch and his collaborators needed more than one inverted microscope to run multiple tests, but wanted to avoid the high costs associated with having more than one system.

Lynch realized that the three Veho VMS-004D 400x USB microscopes (each with 1.3 Mpixel CMOS image sensors) he had purchased could be clamped upside down on a table (for stability) to produce the same images that a far more expensive inverted microscope can. His system, which he calls the Low-Cost Motility Tracking System (LOCOMOTIS), involved creating a 3D model using an open-source software program, followed by its frame and stage construction. For illumination, an external LED strip light was used due to its low heat emission and intensity, which helps reduce stress to the cells.1

Lynch points out that getting the right angle of lighting enabled the LOCOMOTIS system's success. When he turned off the USB microscopes' onboard LED illumination and instead used external illumination, he found that he could see the cells quite clearly—the system allowed him to observe cells that measured about 50 µm long.

The next step for Lynch and his collaborators is to determine additional utilities for LOCOMOTIS, and to lower its cost even further.

1. A. E. Lynch, J. Triajianto, and E. Routledge, PLoS One, 9, 8, e103547 (2014).

Get All the BioOptics World News Delivered to Your Inbox

Subscribe to BioOptics World Magazine or email newsletter today at no cost and receive the latest news and information.

 Subscribe Now
Related Articles

Microscope detects one million-plus biomarkers for sepsis in 30 minutes

A microscope has the potential to simultaneously detect more than one million biomarkers for sepsis at the point of care.

Fluorescence microscopy helps provide new insight into how cancer cells metastasize

By using fluorescence microscopy, scientists have discovered an alternate theory on how some cancer cells metastasize.

Adaptive optics enhances super-resolution microscopy for cell imaging

A new ultra-high resolution nanoscope can take 3D images of an entire cell and its cellular constituents in unprecedented detail.

Fluorescence microscopy approach captures three views of a sample simultaneously

A new fluorescence microscopy approach improves image resolution by acquiring three views of a sample at the same time.


Neuro15 exhibitors meet exacting demands: Part 2

Increasingly, neuroscientists are working with researchers in disciplines such as chemistry and p...

Why be free?

A successful career contributed to keeping OpticalRayTracer—an optical design software program—fr...

LASER Munich 2015 is bio-bent

LASER World of Photonics 2015 included the European Conferences on Biomedical Optics among its si...

White Papers

Understanding Optical Filters

Optical filters can be used to attenuate or enhance an image, transmit or reflect specific wavele...

How can I find the right digital camera for my microscopy application?

Nowadays, image processing is found in a wide range of optical microscopy applications. Examples ...



Twitter- BioOptics World

Copyright © 2007-2016. PennWell Corporation, Tulsa, OK. All Rights Reserved.PRIVACY POLICY | TERMS AND CONDITIONS