NEUROIMAGING/HIGH-RESOLUTION MICROSCOPY: 'Transparentizing' method enables quick whole-brain imaging at single-cell resolution

Imaging of whole brains at single-cell resolution normally involves not only preparing a highly transparent sample (to minimize light scattering), but also imaging fluorescence-tagged neurons at different slices to produce a 3D representation. Such methods, however, limit the study of how phenomena at the cellular scale correlate with activity at the organism level. Researchers at the RIKEN Quantitative Biology Center (Wako, Japan) have addressed this problem with a new high-throughput method that they call clear, unobstructed brain imaging cocktails and computational analysis (CUBIC).1 It offers unprecedented rapid, high-resolution whole-brain imaging and a simple protocol to clear and "transparentize" the brain sample using aminoalcohols.

Thanks to low quenching, CUBIC is compatible with many fluorescent probes, allowing for longer wavelengths and reducing concern for scattering when imaging the whole brain while inviting multicolor imaging. This image of a marmoset brain was created using the CUBIC method
Thanks to low quenching, CUBIC is compatible with many fluorescent probes, allowing for longer wavelengths and reducing concern for scattering when imaging the whole brain while inviting multicolor imaging. This image of a marmoset brain was created using the CUBIC method.

The team tested CUBIC, in conjunction with light-sheet fluorescence microscopy, for quick imaging of different size mammalian brains, including mouse and primate, and for capturing spatial-temporal details of gene expression patterns in the hypothalamic circadian rhythm center. By combining images taken from opposite directions, they were able to generate whole-brain images and directly compare brains in different environmental conditions.

CUBIC provides information on previously unattainable 3D gene expression profiles and neural networks at the systems level. Because of its rapid and high-throughput imaging, CUBIC offers opportunity to analyze localized effects of genomic editing. It also is expected to identify neural connections at the whole-brain level.

In the future, explains center director Hiroki Ueda, MD, Ph.D., the research team would like to apply CUBIC to whole-body imaging at single-cell resolution.

1. E. A. Susaki, Cell, 157, 3, 726–739 (2014).

Get All the BioOptics World News Delivered to Your Inbox

Subscribe to BioOptics World Magazine or email newsletter today at no cost and receive the latest news and information.

 Subscribe Now
Related Articles

New bioimaging technique offers clear view of nervous system

Scientists at Ludwig-Maximilians University have developed a technique for turning the body of a deceased rodent entirely transparent, revealing the central nervous system in unprecedented clarity....

Eye test that pairs two in vivo imaging methods may detect Parkinson's earlier

A low-cost, noninvasive eye test pairs two in vivo imaging methods to help detect Parkinson's before clinical symptoms appear.

New lenses improve two-photon microscopy to image larger area of neuronal activity

By building on two-photon microscopy with new lenses, neuroscientists can better understand the behavior of neurons in the brain.

Optogenetics helps identify neurons that play important role in fear learning

Optogenetics helped to discover the process responsible for persistent reactions to trauma-associated cues.

BLOGS

Neuro15 exhibitors meet exacting demands: Part 2

Increasingly, neuroscientists are working with researchers in disciplines such as chemistry and p...

Why be free?

A successful career contributed to keeping OpticalRayTracer—an optical design software program—fr...

LASER Munich 2015 is bio-bent

LASER World of Photonics 2015 included the European Conferences on Biomedical Optics among its si...

White Papers

Understanding Optical Filters

Optical filters can be used to attenuate or enhance an image, transmit or reflect specific wavele...

How can I find the right digital camera for my microscopy application?

Nowadays, image processing is found in a wide range of optical microscopy applications. Examples ...

CONNECT WITH US

            

Twitter- BioOptics World

Copyright © 2007-2016. PennWell Corporation, Tulsa, OK. All Rights Reserved.PRIVACY POLICY | TERMS AND CONDITIONS