MULTIPHOTON MICROSCOPY: Signaling of thousands of neurons imaged at 250 fps in 3-D

University of California Los Angeles (UCLA) physics professor Katsushi Arisaka thinks that he and his neuroscience colleagues have created the world's fastest two-photon excitation microscope for three-dimensional imaging in-vivo. The work grew from efforts to understand brain disorders. While it is possible to visualize damage resulting from stroke or cancer, disorders such as autism, schizophrenia and learning impairments show no physical signs—and suspicions that these disorders may result from intercellular miscommunication need confirmation. The researchers' collaboratively designed, noninvasive, ultra-high-speed optical microscope can record in real time the firing of thousands of individual neurons in the brain as they communicate, or miscommunicate, with each other.

Click to Enlarge
Physics and neuroscience researchers at UCLA have created what may be the world's fastest two-photon excitation microscope for in-vivo 3-D imaging.

The new approach overcomes limitations of calcium imaging, by capturing cell activity deep in the cortex at a high rate of speed. In fact, the search for speed drove Carlos Portera-Cailliau, assistant professor of neurology and neurobiology, to seek collaboration with Arisaka and graduate student Adrian Cheng. The researchers modified two-photon laser-scanning microscopes to image fluorescent calcium dyes inside the neurons, and came up with a way to split the main laser beam into four smaller beamlets. This spatio-temporal excitation-emission multiplexing (STEM) approach allowed them to record four times as many brain cells as their earlier version, or four times faster. In addition, they used a different beam to record neurons at different depths inside the brain, giving a 3-D effect, which had not been done previously. While most video cameras capture 30 frames per second (fps), the team is able to capture 250 fps and is working to achieve even greater speeds.

Their research appears in the journal Nature Methods.

More BioOptics World Current Issue Articles
More BioOptics World Archives Issue Articles

Get All the BioOptics World News Delivered to Your Inbox

Subscribe to BioOptics World Magazine or email newsletter today at no cost and receive the latest news and information.

 Subscribe Now
Related Articles

New bioimaging technique offers clear view of nervous system

Scientists at Ludwig-Maximilians University have developed a technique for turning the body of a deceased rodent entirely transparent, revealing the central nervous system in unprecedented clarity....

Eye test that pairs two in vivo imaging methods may detect Parkinson's earlier

A low-cost, noninvasive eye test pairs two in vivo imaging methods to help detect Parkinson's before clinical symptoms appear.

New lenses improve two-photon microscopy to image larger area of neuronal activity

By building on two-photon microscopy with new lenses, neuroscientists can better understand the behavior of neurons in the brain.

Optogenetics helps identify neurons that play important role in fear learning

Optogenetics helped to discover the process responsible for persistent reactions to trauma-associated cues.

BLOGS

Neuro15 exhibitors meet exacting demands: Part 2

Increasingly, neuroscientists are working with researchers in disciplines such as chemistry and p...

Why be free?

A successful career contributed to keeping OpticalRayTracer—an optical design software program—fr...

LASER Munich 2015 is bio-bent

LASER World of Photonics 2015 included the European Conferences on Biomedical Optics among its si...

White Papers

Understanding Optical Filters

Optical filters can be used to attenuate or enhance an image, transmit or reflect specific wavele...

How can I find the right digital camera for my microscopy application?

Nowadays, image processing is found in a wide range of optical microscopy applications. Examples ...

CONNECT WITH US

            

Twitter- BioOptics World