Confocal microscopy method discovers how stress wakes up sleeping herpes viruses

Using a confocal microscopy technique, researchers at the University of Helsinki (Finland) and collaborators identified a mechanism by which stress conditions favor the lytic reactivation of the Kaposi's sarcoma associated herpes virus (KSHV) and ensure the efficient production of progeny viruses. KSHV is the etiological agent for Kaposi's sarcoma and primary effusion lymphoma (PEL), an aggressive form of lymphoma with reported median survival time shorter than six months after diagnosis.

Related: Super-resolution microscopy unravels inner structure of herpes simplex virus

Hiding their DNA genome inside the nucleus of the infected cells, herpes viruses establish a lifelong infection in humans. Not well-defined stress conditions are known to wake up these parasites from their dormancy—the latent phase—and reactivate the production of new viral progeny, eventually causing cell death by lysis (the lytic phase). This process allows the virus to multiply and spread usually through lesions of the skin, as in the case of the well known herpes simplex virus. Unlike herpes simplex, however, some other herpes viruses can cause cancer, as they are oncogenic.

The study, combining confocal microscopy and genetic manipulation, shows that a variety of chemical stresses all lead to the activation of a set of cellular "stress sensor" proteins—like p53 and p21clip—that in the attempt of rescuing the cell from the exogenous stress slow down cell proliferation. This process seems to create an environment that favors the production of viral lytic genes, which a few hours after reactivation lead to massive damage of the cellular DNA and arrest the cell division cycle in a stage known as Gap-2 phase or G2. In this status, cells are kept alive by viral proteins, and all cellular nutrients and resources are redirected to the assembly of thousands of new virions.

Herpes viruses reactivate in response to stress. Now, the researchers have found an important link between cellular stress responses, cell cycle regulation, and virus reactivation. (Image courtesy of the Ojala Lab/G Balistreri)

P21clip has a critical role in maintaining cells in the G2-arrested state, the research shows, as removal of this protein by genetic manipulation restored cell division in cells undergoing lytic replication. Importantly, reactivation of cell cycle progression coincided with a reduction in the kinetics of viral lytic gene expression, thus demonstrating that the efficiency of KSHV lytic replication depends on the inhibition of cell division.

This finding provides an important direct link between cellular stress responses, cell cycle regulation, and virus reactivation. Mutations in TP53 gene are rarely found in KSHV-associated malignancies. Therefore, the work now provides a mechanistic explanation as to why the virus has evolved to retain active p53.

The researchers believe that understanding the molecular events that connect cellular stresses with herpes virus reactivation will lead to the development of new antiviral strategies that can be used to treat human cancers caused by oncogenic herpes viruses, such as primary effusion lymphoma, for which there is currently no cure.

Full details of the work appear in the journal PLoS Pathogens; for more information, please visit http://dx.doi.org/10.1371/journal.ppat.1005424.

Get All the BioOptics World News Delivered to Your Inbox

Subscribe to BioOptics World Magazine or email newsletter today at no cost and receive the latest news and information.

 Subscribe Now
Related Articles

New bioimaging technique offers clear view of nervous system

Scientists at Ludwig-Maximilians University have developed a technique for turning the body of a deceased rodent entirely transparent, revealing the central nervous system in unprecedented clarity....

Fluorescent jellyfish proteins light up unconventional laser

Safer lasers to map your cells could soon be in the offing -- all thanks to the humble jellyfish. Conventional lasers, like the pointer you might use to entertain your cat, produce light by emittin...

Microscope detects one million-plus biomarkers for sepsis in 30 minutes

A microscope has the potential to simultaneously detect more than one million biomarkers for sepsis at the point of care.

Eye test that pairs two in vivo imaging methods may detect Parkinson's earlier

A low-cost, noninvasive eye test pairs two in vivo imaging methods to help detect Parkinson's before clinical symptoms appear.

BLOGS

Neuro15 exhibitors meet exacting demands: Part 2

Increasingly, neuroscientists are working with researchers in disciplines such as chemistry and p...

Why be free?

A successful career contributed to keeping OpticalRayTracer—an optical design software program—fr...

LASER Munich 2015 is bio-bent

LASER World of Photonics 2015 included the European Conferences on Biomedical Optics among its si...

White Papers

Understanding Optical Filters

Optical filters can be used to attenuate or enhance an image, transmit or reflect specific wavele...

How can I find the right digital camera for my microscopy application?

Nowadays, image processing is found in a wide range of optical microscopy applications. Examples ...

CONNECT WITH US

            

Twitter- BioOptics World