Hat-like molecular cages allow light activation of signaling peptides in vivo

To transport biomaterials that contain peptide-signaling molecules into living animals, Georgia Institute of Technology (Georgia Tech; Atlanta, GA) researchers and colleagues used hat-like molecular cages to cover binding sites on the peptides that are normally recognized by cell receptors. When activated by ultraviolet (UV) light, the cages detach and reveal the peptides in vivo. If their method could be made to work in humans, it could help provide more precise timing for processes essential to regenerative medicine, cancer treatment, immunology, stem cell growth, and other areas.

Related: Light now able to activate cell surface receptors, with promising implications

“Many biological processes involve complex cascades of reactions in which the timing must be very tightly controlled,” says Andrés García, a Regents Professor in the George W. Woodruff School of Mechanical Engineering at Georgia Tech and principal investigator for the project. “Until now, we haven’t had control over the sequence of events in the response to implanted materials. But with this technique, we can deliver a drug or particle with its signal in the ‘off’ position, then use light to turn the signal ‘on’ precisely when needed.”

Researchers have developed a technique for activating biological signals through the skin of a living animal using light
Researchers have developed a technique for activating biological signals through the skin of a living animal using light. Professor Andrés García, shown here in a Georgia Tech laboratory, is the project’s principal investigator. (Photo credit: Nicole Cappello)

During the five-year project, the research team—which included Ted Lee and Jose Garcia from Georgia Tech and Aranzazu del Campo from the Max Planck Institute—modified peptides that normally trigger cell adhesion to present the molecular cage to disguise them. They showed that disguised peptides introduced into animal models on biomaterials could trigger cell adhesion, inflammation, fibrous encapsulation, and vascularization responses when activated by light. They also showed that the location and timing of activation could be controlled inside the animal by simply shining light through the skin.

The work involved numerous controls to ensure that the triggering observed by the researchers was actually done by exposure of the peptides—not the light, or the removal of the protective cage. The researchers also had to demonstrate that the “hats” were stable enough that they didn’t come off spontaneously, but only when the link between the molecular cage and the peptide was severed by the UV light.

Researchers have developed a technique for activating biological signals through the skin of a living animal using light
Researchers have developed a technique for activating biological signals through the skin of a living animal using light. In this illustration, ultraviolet light is shining through a pattern, initiating fluorescence in the biomaterial implanted in a biomaterial located under the skin of a living animal. (Image courtesy of the García Laboratory at Georgia Tech)

Among the experiments was use of the peptide to attract cells that would attach themselves to the biomaterial. “We showed that if we left the hat on, there would be few cells attracted to the material, García says. “But when we take the hat off, we recruited a lot of cells to the material. That shows we can activate the peptide, and that the activation has a biological consequence.”

Another experiment showed that the timing of peptide activation could affect the quantity of fibrosis, an immune system response that builds a protective capsule around an implanted biomaterial. By delaying the exposure of the peptides until after the bulk of the inflammation reaction had taken place, the thickness of the fibrosis capsule was significantly reduced, allowing it to be better incorporated into the body.

In another experiment, the researchers showed that removing the hats could trigger the growth of blood vessels into the material. This vascularization is critical in regenerative medicine, but must take place at the right time to be successful.

“We showed that if you keep the hat on, you get no vessel in-growth into the material,” explains García. “But if we turn on the light, we get growth of new blood vessels into the material. We can control what happens and when it happens by when we expose the protective cages to light.”

In the future, photochemists at the Max-Planck Institute will be working on alternative cages that would be triggered by different wavelengths of light. As much as 90 percent of the UV light used in the experiments was lost in passing through the skin of the animal model, limiting the use of that wavelength to locations immediately below the skin.

Development of alternate molecular cages could allow sequential activation by light, and light activation of molecules at locations deeper inside the body.

Full details of the work appear in the journal Nature Materials; for more information, please visit http://dx.doi.org/10.1038/nmat4157.

-----

Follow us on Twitter, 'like' us on Facebook, connect with us on Google+, and join our group on LinkedIn

Subscribe now to BioOptics World magazine; it's free!

Get All the BioOptics World News Delivered to Your Inbox

Subscribe to BioOptics World Magazine or email newsletter today at no cost and receive the latest news and information.

 Subscribe Now
Related Articles

New bioimaging technique offers clear view of nervous system

Scientists at Ludwig-Maximilians University have developed a technique for turning the body of a deceased rodent entirely transparent, revealing the central nervous system in unprecedented clarity....

Fluorescent jellyfish proteins light up unconventional laser

Safer lasers to map your cells could soon be in the offing -- all thanks to the humble jellyfish. Conventional lasers, like the pointer you might use to entertain your cat, produce light by emittin...

Eye test that pairs two in vivo imaging methods may detect Parkinson's earlier

A low-cost, noninvasive eye test pairs two in vivo imaging methods to help detect Parkinson's before clinical symptoms appear.

Fluorescence microscopy helps provide new insight into how cancer cells metastasize

By using fluorescence microscopy, scientists have discovered an alternate theory on how some cancer cells metastasize.

BLOGS

Neuro15 exhibitors meet exacting demands: Part 2

Increasingly, neuroscientists are working with researchers in disciplines such as chemistry and p...

Why be free?

A successful career contributed to keeping OpticalRayTracer—an optical design software program—fr...

LASER Munich 2015 is bio-bent

LASER World of Photonics 2015 included the European Conferences on Biomedical Optics among its si...

White Papers

Understanding Optical Filters

Optical filters can be used to attenuate or enhance an image, transmit or reflect specific wavele...

How can I find the right digital camera for my microscopy application?

Nowadays, image processing is found in a wide range of optical microscopy applications. Examples ...

CONNECT WITH US

            

Twitter- BioOptics World

Copyright © 2007-2016. PennWell Corporation, Tulsa, OK. All Rights Reserved.PRIVACY POLICY | TERMS AND CONDITIONS