Ultrafast nonlinear microscopy can ID breast cancer tumors in real time

Recognizing that almost half of patients undergoing breast-conserving cancer surgery have to undergo additional procedures due to cancerous tumor being left behind, a team of researchers has found that an ultrafast nonlinear microscopy technique can identify breast tumor margins during surgery in real time. The work's implications could mean more accurate tumor removal and reduction in additional surgeries.

Related: Photonics answers important questions in surgical guidance

The research team comprises members from the Massachusetts Insitute of Technology (MIT), Harvard Medical School (both in Cambridge, MA), and Thorlabs (Newton, NJ), and was led by James G. Fujimoto, the Elihu Thomson Professor of Electrical Engineering at MIT. The team's microscopy technique involved a 100 fs tunable Ti:sapphire laser at 740 nm with a 76 MHz repetition rate and a commercially available nonlinear microscope, which yielded high-resolution images of morphological changes associated with breast cancer, like collagen reorganization, in breast tissue samples during surgery. Using the technique, the team was able to overcome limited field of view in high-magnification imaging with scanning and field mosaicking, resulting in subcellular resolution in square-centimeter-sized specimens.

The team's study involved 179 fresh surgical specimens from 50 patients for tumor margin assessment. When compared with traditional paraffin or frozen-section analyses, the microscopy technique achieved 94 percent overall accuracy for identifying tumor borders for breast cancer in situ.

The researchers say that additional utilities for the technique could be for lung, thyroid, and head and neck cancer surgery assessments.

Full details of the team's work appear in the Proceedings of the National Academy of Sciences; for more information, please visit http://dx.doi.org/10.1073/pnas.1416955111.

-----

Follow us on Twitter, 'like' us on Facebook, connect with us on Google+, and join our group on LinkedIn

Subscribe now to BioOptics World magazine; it's free!

Get All the BioOptics World News Delivered to Your Inbox

Subscribe to BioOptics World Magazine or email newsletter today at no cost and receive the latest news and information.

 Subscribe Now
Related Articles

New bioimaging technique offers clear view of nervous system

Scientists at Ludwig-Maximilians University have developed a technique for turning the body of a deceased rodent entirely transparent, revealing the central nervous system in unprecedented clarity....

Fluorescent jellyfish proteins light up unconventional laser

Safer lasers to map your cells could soon be in the offing -- all thanks to the humble jellyfish. Conventional lasers, like the pointer you might use to entertain your cat, produce light by emittin...

Microscope detects one million-plus biomarkers for sepsis in 30 minutes

A microscope has the potential to simultaneously detect more than one million biomarkers for sepsis at the point of care.

Eye test that pairs two in vivo imaging methods may detect Parkinson's earlier

A low-cost, noninvasive eye test pairs two in vivo imaging methods to help detect Parkinson's before clinical symptoms appear.

BLOGS

Neuro15 exhibitors meet exacting demands: Part 2

Increasingly, neuroscientists are working with researchers in disciplines such as chemistry and p...

Why be free?

A successful career contributed to keeping OpticalRayTracer—an optical design software program—fr...

LASER Munich 2015 is bio-bent

LASER World of Photonics 2015 included the European Conferences on Biomedical Optics among its si...

White Papers

Understanding Optical Filters

Optical filters can be used to attenuate or enhance an image, transmit or reflect specific wavele...

How can I find the right digital camera for my microscopy application?

Nowadays, image processing is found in a wide range of optical microscopy applications. Examples ...

CONNECT WITH US

            

Twitter- BioOptics World

Copyright © 2007-2016. PennWell Corporation, Tulsa, OK. All Rights Reserved.PRIVACY POLICY | TERMS AND CONDITIONS