Optogenetics confirms that strengthened connections between neurons fuel memories

Using optogenetics, University of California, San Diego (UCSD) scientists inactivated and then reactivated a memory in genetically engineered rats. The study, supported by the National Institutes of Health (NIH; Bethesda, MD), is the first cause-and-effect evidence that strengthened connections between neurons are what fuel a memory.

Related: Light triggering demonstrates that memory engrams are physical phenomena

Optogenetics uses low-level light to selectively activate proteins administered to live tissues. It adapts the same cellular machinery that allows primitive organisms like algae to be controlled by light from the sun to control specific brain circuit components instantly with a laser—even in a behaving animal.

"Our results add to mounting evidence that the brain represents a memory by forming assemblies of neurons with strengthened connections, or synapses," explains Roberto Malinow, MD, Ph.D., of UCSD, a grantee of NIH's National Institute of Mental Health (NIMH). "Further, the findings suggest that weakening synapses likely disassembles neuronal assemblies to inactivate a memory."

"Beyond potential applications in disorders of memory deficiency, such as dementia, this improved understanding of how memory works may hold clues to taking control of runaway emotional memories in mental illnesses, such as post-traumatic stress disorder," said NIMH director Thomas R. Insel, MD.

Neuroscientists have long suspected that strengthened connections between neurons—called long-term potentiation (LTP)—underlies memory formation. But proof had remained elusive—until now. The research team proved it by detecting LTP when forming a memory, removing the memory with a process known to reverse LTP, and then bringing the memory back via LTP—all by modifying the strength of synapses in a memory circuit.
In conventional rodent fear conditioning experiments, a tone is paired with a foot shock to induce a fear memory of the tone.  If the memory is active, the animal freezes and shows reduced reward-seeking behaviors when it hears the tone. Instead of the tone, Malinow's team paired the shock with direct optogenetic stimulation, lighting up a specific group of neurons in a known auditory fear memory circuit.

Such precise targeting wasn't possible with earlier electrical stimulation techniques. "It is just a jungle in the brain—too many nerve cells coming through in any one place," explains Malinow.

By varying the pattern of optogenetic stimulation, the researchers were able to strengthen connections between neurons in the circuit by promoting LTP or weaken the connections by promoting a countervailing process called long-term depression (LTD). This made it possible to readily form a fear memory, remove it, and then bring it back.

Moreover, upon closer optogenetic probing in postmortem brains, the targeted circuit neurons showed telltale changes in sensitivity of brain chemical messenger systems. These changes confirmed the hypothesized role of strengthening and weakening of synaptic connections in the switching on-and-off of the memory.

"We have shown that the damaging products that build up in the brains of Alzheimer's disease patients can weaken synapses in the same way that we weakened synapses to remove a memory," says Malinow. "So this line of research could suggest ways to intervene in the process."

"In addition to eliminating any doubt about a link between LTP/LTD with memories, this work highlights the staggering potential of precision targeting and circuit manipulation for alleviating maladaptive memories," says project officer Chiiko Asanuma, Ph.D., of the NIMH Division of Neuroscience and Basic Behavioral Science.

"This work provides a nice demonstration of how the field of neuroscience is being transformed by the types of technologies that are at the heart of President Obama's BRAIN Initiative," says Edmund Talley, Ph.D., program director at NIH's National Institute on Neurological Disorders and Stroke (NINDS).

Full details of the work appear in the journal Nature; for more information, please visit http://www.nature.com/nature/journal/vaop/ncurrent/full/nature13294.html.


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