Spectroscopy method IDs, quantifies molecular species in real time

A team of scientists at the Laser Spectroscopy Division of the Max Planck Institute of Quantum Optics (MPQ; Garching, Germany), Ludwig Maximilian University (LMU; Munich, Germany), and the Institut des Sciences Moléculaires d’Orsay (France) has developed a real-time identification and quantification method for molecules. The work has implications in areas such as bioimaging, where real-time recording of molecular spectra is important.

Related: Wavelength-modulated Raman spectroscopy enables tissue assessment in real time

Dual-comb spectroscopy has demonstrated exciting potential for ultra-rapid recording of complex molecular spectra. When compared to state-of-the-art instrumentation used in Fourier transform spectroscopy, it shortens recording times from seconds to microseconds. Dual-comb spectroscopy without moving parts harnesses two laser frequency combs (coherent sources that emit a regular train of ultrashort pulses) to perform interferometric measurements, but it is difficult to synchronize the two lasers within the required precision. Recognizing this, the research team developed a new approach that enabled them to use unstabilized free-running femtosecond lasers without sacrificing performance. By generating proper clock signals, they compensated for laser short-term instabilities by electronic signal processing only.

Related: Ultrafast pulses for precision procedures

Portion of a dual-comb real-time absorption spectrum of acetylene in the near-infrared (NIR) region
Portion of a dual-comb real-time absorption spectrum of acetylene in the near-infrared (NIR) region. While the spectrum without the adaptive sampling (blind sampling) is strongly distorted, the adaptive spectrum accurately reveals the molecular profiles. (Image courtesy of MPQ/Laser Spectroscopy Division)

The possibility of using femtosecond lasers without any sophisticated stabilization scheme considerably eases the implementation of a dual-comb spectrometer. "It should facilitate applications to real-time sensing," comments Takuro Ideguchi, who just completed his doctoral dissertation partly based on this experiment. "Dual-comb spectroscopy holds much promise for new approaches to molecular physics and our scheme of adaptive sampling is a key to their efficient implementation. Enhanced sensitivity for weak concentrations is within reach with the development of mid-infrared frequency combs, while nonlinear dual-comb spectroscopy provides intriguing prospects for applications ranging from bioimaging to precision spectroscopy."

Full details of the work appear in the journal Nature Communications; for more information, please visit http://dx.doi.org/10.1038/ncomms4375.


Follow us on Twitter, 'like' us on Facebook, and join our group on LinkedIn

Subscribe now to BioOptics World magazine; it's free!

Get All the BioOptics World News Delivered to Your Inbox

Subscribe to BioOptics World Magazine or email newsletter today at no cost and receive the latest news and information.

 Subscribe Now
Related Articles

FDA authorizes emergency use of Zika virus molecular detection assay

The xMAP MultiFLEX Zika RNA assay combines optofluidics and digital signal processing to detect Zika virus in vitro.

Merz acquires laser tattoo removal device maker ON Light Sciences

Merz North America has acquired ON Light Sciences, which develops technologies to enhance laser-based dermatology procedures.

Shortwave-infrared device could improve ear infection diagnosis

An otoscope-like device that could improve ear infection diagnosis uses shortwave-infrared light instead of visible light.

Laser therapy extracts rare tumor that grew human hair, skin in boy's skull

About four years ago, a tumor comprised of human skin, hair, bone and cartilage was fast-growing inside a Ramsey, MN, 10-year-old youth's brain.

Neuro15 exhibitors meet exacting demands: Part 2

Increasingly, neuroscientists are working with researchers in disciplines such as chemistry and p...

Why be free?

A successful career contributed to keeping OpticalRayTracer—an optical design software program—fr...

LASER Munich 2015 is bio-bent

LASER World of Photonics 2015 included the European Conferences on Biomedical Optics among its si...

White Papers

Understanding Optical Filters

Optical filters can be used to attenuate or enhance an image, transmit or reflect specific wavele...

How can I find the right digital camera for my microscopy application?

Nowadays, image processing is found in a wide range of optical microscopy applications. Examples ...



Twitter- BioOptics World

Copyright © 2007-2016. PennWell Corporation, Tulsa, OK. All Rights Reserved.PRIVACY POLICY | TERMS AND CONDITIONS