FRET-based biosensor can screen for potential analgesic drugs

A team of researchers from the Neuropharmacology and Pain Research Group of the Bellvitge Biomedical Research Institute (IDIBELL), the University of Barcelona (UB), and ESTEVE (all in Barcelona, Spain) has developed a fluorescence resonance energy transfer (FRET)-based biosensor that can determine drugs' analgesic potential before being tested in animal models. The finding could save time and money in research on new, effective pain treatments.

Related: The many approaches and applications of biosensing

Related: CIMIT enables acceleration of fluorescent drug-screening device

In a study led by Francisco Ciruela, senior lecturer of the Department of Pathology and Experimental Therapy at UB and a researcher in the Neuropharmacology and Pain Research Group, the researchers' FRET-based biosensor allowed them to classify—in cells in culture—ligands in sigma-1 receptor into agonists and antagonists.

The experiments established a direct correlation between the biosensor's FRET signal in response to the drugs and their analgesic effect in a pain animal model. Sigma-1 agonists reduce FRET signal and have low analgesic effects, whereas sigma-1 antagonists increase FRET signal and have high analgesic effects in pain animal models.

"Thanks to this well-defined pattern, we can predict the analgesic behavior of a molecule," explains Ciruela. "Therefore, if FRET signal is reduced—that is, if it is an agonist—it won’t have analgesic effects on the pain animal model. However, if FRET signal is increased—in other words, if it is an antagonist—it will have maximum analgesic effects and, therefore, it will be a suitable candidate to be tested in vivo."

Ciruela points out that the new technology has already been patented, with the expectation that it will be soon lead to a high-throughput screening method.

Full details of the work appear in the Journal of Medicinal Chemistry; for more information, please visit http://pubs.acs.org/doi/abs/10.1021/jm401529t.
   
-----

Follow us on Twitter, 'like' us on Facebook, and join our group on LinkedIn

Subscribe now to BioOptics World magazine; it's free!

POST A COMMENT

Get All the BioOptics World News Delivered to Your Inbox

Subscribe to BioOptics World Magazine or email newsletter today at no cost and receive the latest news and information.

 Subscribe Now
Related Articles

Implantable polymer fiber emits fluorescence to reveal how cells grow

Fiber from a semiconducting polymer can emit fluorescence, which makes it possible to follow the growth of cells inside living tissue.

New super-resolution microscopy method is possible with single objective

A newly developed super-resolution microscopy method requires only a single objective and works with standard microscopy systems.

Imaging system combines five molecular imaging techniques

A newly developed system combines five molecular imaging techniques for multimodal imaging of both tissue models and live subjects.

Silicone microspheres show promise for medical imaging, targeted drug delivery

Chemists have produced silicone microspheres that could enable next-generation medical imaging and targeted medicine.

Low-light CMOS biosensor enables detection of four copies of pathogen DNA per sample

Anitoa Systems has demonstrated handheld, real-time qPCR using its ultra-low-light CMOS biosensor.

Silver nanoclusters inside synthetic DNA have utility in bioimaging

A team of researchers has created nanoscale silver clusters with unique fluorescence properties important for bioimaging.

GWU installs correlative microscopy system at its new research facility

George Washington University will install a correlative light and electron microscopy system at its Science and Engineering Hall.

Ultrafast laser technique enables nondestructive 3D imaging of cells

An ultrafast laser technique can achieve micron resolution of single cells, imaging their interiors in slices separated by 150 nm.

Super-resolution microscopy unravels inner structure of herpes simplex virus

A team of researchers has developed a technique that allows super-resolution microscopy to be used as a structural tool for the study of viruses.

BLOGS

LASER Munich 2015 is bio-bent

LASER World of Photonics 2015 included the European Conferences on Biomedical Optics among its si...

Single-molecule tracking promises discoveries, cures

New ways of imaging individual proteins and lipids will ultimately change science and medicine.

Growth in lasers for medicine

At the Lasers and Photonics Marketplace Seminar during SPIE Photonics West 2015, analyst Allen No...

Most Popular Articles


CONNECT WITH US

            

Twitter- BioOptics World

Copyright © 2007-2015. PennWell Corporation, Tulsa, OK. All Rights Reserved.PRIVACY POLICY | TERMS AND CONDITIONS