NIR-fluorescing dye enables improved brain study

A team of researchers from the Laboratoire de Chimie at Claude Bernard University Lyon 1 (Lyon, France), the Institut des Neurosciences Grenoble (Grenoble, France), and the Interdisciplinary Chemistry: Synthesis, Analysis, Modeling (CEISAM) lab at the University of Nantes (Nantes, France) have developed a dye that fluoresces in the near-infrared (NIR) and can pass through the skin. The chromophore they synthesized, dubbed Lem-PHEA, opens up significant prospects for better observing the brain and understanding how it works.

Image of the cerebral vascular system of a mouse obtained by 3D two-photon microscopy with addition of Lem-PHEA
Image of the cerebral vascular system of a mouse obtained by 3D two-photon microscopy with addition of Lem-PHEA. (Image courtesy of B. van der Sanden and F. Appaix, Institut des Neurosciences de Grenoble)

Related: New approach overcomes limitations of two-photon dual-color imaging

To obtain images of the vascular system of a mouse brain, it is necessary to use a fluorescent dye that combines NIR luminescence, solubility in biological media, low cost, non-toxicity, and utility for 3D two-photon microscopy. Lem-PHEA combines these properties; when injected into the blood vessels of a mouse, it reveals details of a rodent's vascular system with unprecedented detail, thanks to a considerably enhanced fluorescence compared to conventional dyes such as Rhodamine-B and cyanine derivatives. What's more, Lem-PHEA is easily eliminated by the kidneys.

Their work has been published online in the journal Chemical Science; for more information, please visit


Follow us on Twitter, 'like' us on Facebook, and join our group on LinkedIn

Subscribe now to BioOptics World magazine; it's free!

Get All the BioOptics World News Delivered to Your Inbox

Subscribe to BioOptics World Magazine or email newsletter today at no cost and receive the latest news and information.

 Subscribe Now
Related Articles

New bioimaging technique offers clear view of nervous system

Scientists at Ludwig-Maximilians University have developed a technique for turning the body of a deceased rodent entirely transparent, revealing the central nervous system in unprecedented clarity....

Fluorescent jellyfish proteins light up unconventional laser

Safer lasers to map your cells could soon be in the offing -- all thanks to the humble jellyfish. Conventional lasers, like the pointer you might use to entertain your cat, produce light by emittin...

Fluorescence microscopy helps provide new insight into how cancer cells metastasize

By using fluorescence microscopy, scientists have discovered an alternate theory on how some cancer cells metastasize.

In vivo imaging method visualizes bone-resorbing cell function in real time

In vivo imaging can visualize sites where osteoclasts (bone-resorbing cells) were in the process of resorbing bone.


Neuro15 exhibitors meet exacting demands: Part 2

Increasingly, neuroscientists are working with researchers in disciplines such as chemistry and p...

Why be free?

A successful career contributed to keeping OpticalRayTracer—an optical design software program—fr...

LASER Munich 2015 is bio-bent

LASER World of Photonics 2015 included the European Conferences on Biomedical Optics among its si...

White Papers

Understanding Optical Filters

Optical filters can be used to attenuate or enhance an image, transmit or reflect specific wavele...

How can I find the right digital camera for my microscopy application?

Nowadays, image processing is found in a wide range of optical microscopy applications. Examples ...



Twitter- BioOptics World

Copyright © 2007-2016. PennWell Corporation, Tulsa, OK. All Rights Reserved.PRIVACY POLICY | TERMS AND CONDITIONS