Light sources for quantitative fluorescence imaging by 89 North

89 North PhotoFluor II and PhotoFluor II NIR light sources for quantitative fluorescence imaging

The PhotoFluor II and PhotoFluor II NIR light sources for quantitative fluorescence imaging from 89 North (Burlington, VT) pair a self-aligning, 200 W metal-halide lamp with a sputtered, infrared (IR)-blocking filter to enable transmission in the UV and NIR, respectively. PhotoFluor II works in the 340–650 nm wavelength range, while PhotoFluor II NIR works from 360 to 800 nm. Both light sources accommodate imaging of several fluorescence probes, including—but not limited to—green fluorescent protein (GFP), mCherry, and Texas Red.

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PhotoFluor® II and PhotoFluor II NIR
high powered, ULTRASTABLE light sources for quantitative fluorescence imaging

more power where you need it
The PhotoFluor® II and the PhotoFluor II NIR combines a powerful self-aligning 200W metal-halide lamp with a sputtered IR-blocking filter to deliver exceptional output intensity in the visible spectrum.

The PhotoFluor II has enhanced transmission in the UV while the PhotoFluor II NIR has enhanced NIR transmission allowing the user to focus on specific applications while minimizing damage to the Liquid Light Guide seen in other white light systems.

stable output
Fluorescence imaging techniques have moved away from simple qualitative analyses to more rigorous quantitative experimentation. To accurately quantify sequential fluorescence images, it is essential that the output of your light source remain constant between exposures. Fluctuations in output intensity impart significant error in your experiments. The PhotoFluor II and the PhotoFluor II NIR offer unsurpassed output stability over both the short and long term, enabling truly quantitative fluorescence imaging.

wavelength range
PhotoFluor II 340nm – 650nm
PhotoFluor II NIR 360nm – 800nm

The PhotoFluor II and PhotoFluor II NIR are well suited for imaging a wide array of fluorescence probes including:

Both PhotoFluor II and PhotoFluor II NIR
CFP, GFP, FITC, YFP, TRITC, mCherry, Texas Red, MitoTracker Red, Di-4-ANNEPS, Fluo-4, Fura Red

PhotoFluor II
Fura-2, Indo-1

PhotoFluor II NIR
Cy7, IRDye 800, AlexaFluor 750, iRFP


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