'Reverse' spectroscopy approach clarifies molecular structure

Optical techniques enable examination of single molecules, but fuzziness caused by effects such as light interference render these images difficult to interpret. Recognizing this, researchers at the University of Twente’s MESA+ Institute for Nanotechnology (Enschede, The Netherlands) adopted a "reverse" approach to spectroscopy, which cleans up images by eliminating background noise.

Rather than starting with a laser beam, the researchers used molecules of interest as their starting point. This radical "reversal" led to a relatively simple modification of conventional coherent anti-Stokes Raman spectroscopy (CARS), which delivered better images. CARS uses lasers to visualize molecules for applications such as medical imaging and food testing, without needing fluorescent labels to make the molecules visible. But background noise complicates the task of interpreting the resultant images. The researchers' new approach—vibrational molecular interferometry (VMI)—eliminates such noise completely, leaving only the "real" image.

With vibrational molecular interferometry (VMI), the background signal in a single-molecule image vanishes
Images of mayonnaise using a) CARS spectroscopy and b) vibrational molecular interferometry (VMI). Below, the intensity along the white line in the picture is given. Using VMI, the background signal vanishes. (Image courtesy of University of Twente MESA+ Institute for Nanotechnology)

The researchers' work has been published in Physical Review Letters; for more information, please visit http://prl.aps.org/abstract/PRL/v107/i25/e253902.

-----

Follow us on Twitter, 'like' us on Facebook, and join our group on LinkedIn

Follow OptoIQ on your iPhone; download the free app here.

Subscribe now to BioOptics World magazine; it's free!

Get All the BioOptics World News Delivered to Your Inbox

Subscribe to BioOptics World Magazine or email newsletter today at no cost and receive the latest news and information.

 Subscribe Now
Related Articles

LuxCath optical tissue characterization catheter enables real-time monitoring during cardiac ablation

A study used optical tissue characterization technology for the first time in procedures to treat arrhythmia patients.

EUV spectral imaging tool can map cell composition in 3D

A newly developed spectral imaging instrument enables observation of how cells respond to new medications at a minute level.

Fluorescence Imaging: Optical filtering basics for life sciences

Optical filters can have a dramatic effect on outcomes in life sciences. These principles demonstrate how next-generation thin film enhances excitation and emission in fluorescence bioimaging syste...

Photoacoustics/Biomedical Imaging: Photoacoustic imaging progresses toward medical diagnostics

Recent technological developments in laser and transducer hardware, contrast agents, and image reconstruction algorithms have helped to advance photoacoustic (or optoacoustic) imaging.  

Translational Research: Bench-to-bedside: Progress, pioneers, and 21st Century Cures

The NIH/SPIE Biophotonics from Bench to Bedside workshop (Sept. 24-25) featured speakers and posters presenting exciting translational research in technologies and applications.

Legislation promises biophotonics opportunities

The 21st Century Cures Act (H.R. 6) was a focal point at the NIH/SPIE Biophotonics from Bench to Bedside workshop.

Zeiss partners with Molecular Imaging Platform at McGill University Health Centre

Zeiss has entered into a partnership with the Research Institute of the McGill University Health Centre's Molecular Imaging Platform.

Biophotonics innovator Ozcan wins International Commission for Optics Prize

UCLA professor and biophotonics innovator Aydogan Ozcan has received the International Commission for Optics (ICO) Prize.

New biosensor provides spatially resolved hydrogen peroxide sensing in cells

A new biosensor is able to show the location of the key cellular signaling chemical inside living cells with high resolution over time.

BLOGS

Neuro15 exhibitors meet exacting demands: Part 2

Increasingly, neuroscientists are working with researchers in disciplines such as chemistry and p...

Why be free?

A successful career contributed to keeping OpticalRayTracer—an optical design software program—fr...

LASER Munich 2015 is bio-bent

LASER World of Photonics 2015 included the European Conferences on Biomedical Optics among its si...

White Papers

Understanding Optical Filters

Optical filters can be used to attenuate or enhance an image, transmit or reflect specific wavele...

How can I find the right digital camera for my microscopy application?

Nowadays, image processing is found in a wide range of optical microscopy applications. Examples ...

CONNECT WITH US

            

Twitter- BioOptics World

Copyright © 2007-2015. PennWell Corporation, Tulsa, OK. All Rights Reserved.PRIVACY POLICY | TERMS AND CONDITIONS