Noninvasive Raman microspectroscopy technique boosts stem cell therapy

Nottingham University's Raman microspectrometer with back-illuminated CCD camera
Taken in Dr. Ioan Notingher's lab at Nottingham University, the custom-built Raman microspectrometer with a cooled, deep-depletion, back-illuminated CCD camera can identify live cardiomyocyte cells within highly heterogeneous cell populations noninvasively.

In stem cell therapy, a problem that exists is controlling the excessive proliferation of cells with unwanted phenotypes after transplantation to prevent tissue overgrowth and tumor formation. And most techniques currently available for characterizing cells are invasive, therefore rendering the cells unusable. Recognizing this, researchers at Nottingham University (Nottingham, England) have developed a noninvasive Raman microspectroscopy (RMS) technique that phenotypically identifies live cardiomyocyte cells within highly heterogeneous cell populations with greater than 96% sensitivity and specificity.

Led by Dr. Ioan Notingher, the team used a back-illuminated CCD camera (from Andor Technology) attached to a purpose-built Raman microspectrometer to record spectra from individual cells derived from micrometric regions of human embryonic stem cells (hESCs). By comparing with matching immunofluorescence images from the same cells, they showed that the Raman spectra correspond to the spatial distribution of biomolecules such as nucleic acids, proteins, lipids and carbohydrates, and that this can be used to discriminate between different cell types.

To yield the shortest possible acquisition times for the Raman spectra, the team's CCD camera enabled measurements of Raman spectra from selected positions in the cells in only 0.5 sec, says Notingher. The camera's detectors work in the 800–900 nm spectral range, thereby avoiding photodamage to the cells. And since RMS has only a minimal background signal from water, it allows repeated observations of viable cells maintained under physiological conditions, he adds.

-----

Follow us on Twitter, 'like' us on Facebook, and join our group on LinkedIn

Follow OptoIQ on your iPhone; download the free app here.

Subscribe now to BioOptics World magazine; it's free!

Get All the BioOptics World News Delivered to Your Inbox

Subscribe to BioOptics World Magazine or email newsletter today at no cost and receive the latest news and information.

 Subscribe Now
Related Articles

Fluorescent jellyfish proteins light up unconventional laser

Safer lasers to map your cells could soon be in the offing -- all thanks to the humble jellyfish. Conventional lasers, like the pointer you might use to entertain your cat, produce light by emittin...

Fluorescence microscopy helps provide new insight into how cancer cells metastasize

By using fluorescence microscopy, scientists have discovered an alternate theory on how some cancer cells metastasize.

In vivo imaging method visualizes bone-resorbing cell function in real time

In vivo imaging can visualize sites where osteoclasts (bone-resorbing cells) were in the process of resorbing bone.

Flow cytometry analyzes cell population to predict cancer immunotherapy response

Flow cytometry helped find that the amount of white blood cells in melanoma tumors can predict response to a cancer therapy.

BLOGS

Neuro15 exhibitors meet exacting demands: Part 2

Increasingly, neuroscientists are working with researchers in disciplines such as chemistry and p...

Why be free?

A successful career contributed to keeping OpticalRayTracer—an optical design software program—fr...

LASER Munich 2015 is bio-bent

LASER World of Photonics 2015 included the European Conferences on Biomedical Optics among its si...

White Papers

Understanding Optical Filters

Optical filters can be used to attenuate or enhance an image, transmit or reflect specific wavele...

How can I find the right digital camera for my microscopy application?

Nowadays, image processing is found in a wide range of optical microscopy applications. Examples ...

CONNECT WITH US

            

Twitter- BioOptics World

Copyright © 2007-2016. PennWell Corporation, Tulsa, OK. All Rights Reserved.PRIVACY POLICY | TERMS AND CONDITIONS