Coherent anti-Stokes Raman spectroscopy kit by EKSPLA

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The CARS Micro-Spectroscopy Kit from EKSPLA (Vilnius, Lithuania) includes the company's PT259 pump laser, CARS spectrometer, and CARS microscope in one system for coherent anti-Stokes Raman spectroscopy (CARS), species-selective microscopy, 3-D sample imaging, and dynamic live cell imaging. Features include a non-photobleaching signal for live cell studies, a broadband tuning range of 700–4000 cm-1, high spatial resolution, and a lower wavelength for minimal fluorescence interference. The kit can transform to a microspectrometer for fluorescence measurements based on two-photon excitation.

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PRESS RELEASE

CARS Micro-Spectroscopy Kit

Coherent anti-Stokes Raman spectroscopy, also called Coherent anti- Stokes Raman scattering spectroscopy (CARS), is a form of spectroscopy used primarily in chemistry, physics and related fields.

Features

  • Minimally invasive technique
  • Non-photobleaching signal for live cell studies
  • Broadband tuning range 700 – 4000 cm-1
  • Higher sensitivity (stronger signals) than spontaneous Raman microscopy – faster, more efficient imaging for real-time analysis
  • CARS signal is at high frequency (lower wavelength) – minimal fluorescence interference
  • High spatial resolution
  • Integrated with Ekspla tunable laser PT259
  • System easy transformable to micro-spectrometer for fluorescence measurements based on two-photon excitation

Applications

  • E-CARS, F-CARS, P-CARS spectroscopy
  • Species selective microscopy
  • 3D sample imaging
  • Dynamic live cell imaging
  • Long-term live cell processes monitoring
  • Non-destructive medical, biological research
  • Your application is welcome…

Coherent anti-Stokes Raman spectroscopy, also called Coherent anti- Stokes Raman scattering spectroscopy (CARS), is a form of spectroscopy used primarily in chemistry, physics and related fields. It is sensitive to the same vibrational signatures of molecules as seen in Raman spectroscopy, typically the nuclear vibrations of chemical bonds. Unlike Raman spectroscopy, CARS employs multiple photons to address the molecular vibrations, and produces a signal in which the emitted waves are coherent with one another. As a result, CARS is orders of magnitude stronger than spontaneous Raman emission. CARS is a third-order nonlinear optical process involving three laser beams: a pump beam of frequency ωpump, a Stokes beam of frequency ωStokes and a probe beam at frequency ωprobe. These beams interact with the sample and generate a coherent optical signal at the anti-Stokes frequency
ωCARS=ωpump-ωStokes+ωprobe.

The CARS signal ωCARS is resonantly enhanced when the difference between the pump ωpump and Stokes ωStokes frequencies matches a vibrational transition ωvib of the molecule.

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Posted by Lee Mather

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