Nanoparticle tracking analysis teams with fluorescence measurement for early disease detection

Researchers from the Nuffield Department of Obstetrics & Gynecology at Oxford University (Oxford, England) recently used a fluorescence nanoparticle tracking analysis (NTA) system from NanoSight (Amesbury, England) to quickly size and phenotype cellular vesicles for use as biomarkers for early disease detection. Their findings overcome previous limitations in the technology available for their measurement.

The NTA system visualizes vesicles by light scattering using a light microscope, records a video, and then tracks the Brownian motion of the individual vesicles via the NTA software, calculating their size and total concentration.

Using human placental vesicles and plasma, the team has demonstrated that NTA can measure cellular vesicles as small as ~50 nm, making it far more sensitive than conventional flow cytometry (lower limit ~300 nm). By combining NTA with fluorescence measurement, vesicles can be labeled with specific antibody-conjugated quantum dots, allowing their phenotype to be determined.

The researchers' work has been published in NanoMedicine, and comprehensive funding for the work was provided by a Wellcome Trust Technology Development, a Wellcome Trust Program Grant and by the Oxford Partnership Comprehensive Biomedical Research Center, with support from the Department of Health's NIHR Biomedical Research Center's funding scheme.


Posted by Lee Mather

Follow us on Twitter, 'like' us on Facebook, and join our group on LinkedIn

Follow OptoIQ on your iPhone; download the free app here.

Subscribe now to BioOptics World magazine; it's free!

Get All the BioOptics World News Delivered to Your Inbox

Subscribe to BioOptics World Magazine or email newsletter today at no cost and receive the latest news and information.

 Subscribe Now
Related Articles

New bioimaging technique offers clear view of nervous system

Scientists at Ludwig-Maximilians University have developed a technique for turning the body of a deceased rodent entirely transparent, revealing the central nervous system in unprecedented clarity....

Fluorescent jellyfish proteins light up unconventional laser

Safer lasers to map your cells could soon be in the offing -- all thanks to the humble jellyfish. Conventional lasers, like the pointer you might use to entertain your cat, produce light by emittin...

Fluorescence microscopy helps provide new insight into how cancer cells metastasize

By using fluorescence microscopy, scientists have discovered an alternate theory on how some cancer cells metastasize.

In vivo imaging method visualizes bone-resorbing cell function in real time

In vivo imaging can visualize sites where osteoclasts (bone-resorbing cells) were in the process of resorbing bone.


Neuro15 exhibitors meet exacting demands: Part 2

Increasingly, neuroscientists are working with researchers in disciplines such as chemistry and p...

Why be free?

A successful career contributed to keeping OpticalRayTracer—an optical design software program—fr...

LASER Munich 2015 is bio-bent

LASER World of Photonics 2015 included the European Conferences on Biomedical Optics among its si...

White Papers

Understanding Optical Filters

Optical filters can be used to attenuate or enhance an image, transmit or reflect specific wavele...

How can I find the right digital camera for my microscopy application?

Nowadays, image processing is found in a wide range of optical microscopy applications. Examples ...



Twitter- BioOptics World

Copyright © 2007-2016. PennWell Corporation, Tulsa, OK. All Rights Reserved.PRIVACY POLICY | TERMS AND CONDITIONS