Semrock long-pass fluorescence filter sets for fluorophore viewing

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The BrightLine long-pass fluorescence filter sets from Semrock (Rochester, NY) capture fluorescence signal for low fluorophore concentration applications, when signal-to-noise ratio is not as critical, and for viewing fluorophores such as DAPI, GFP, and red fluorescent proteins with wide emission spectra or large wavelength shift between excitation and emission. All offer >93% average transmission over the passband and extremely steep edges for capturing fluorescence close to the excitation band.

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PRESS RELEASE

New Long-pass Fluorescence Filter Sets from Semrock

ROCHESTER, N.Y., September 24, 2010 – Semrock has just released four new BrightLine® long-pass fluorescence filter sets to enhance its full line of established high-performance bandpass filter sets.  With these long-pass sets more fluorescence signal can be captured for low fluorophore concentration applications and when signal-to-noise ratio is not as critical.  They are also well suited for viewing fluorophores with very wide emission spectra or with a large wavelength shift between the excitation and emission wavelengths.   The new filter sets are targeted at imaging the most popular fluorophores including DAPI, GFP, and red fluorescent proteins such as mCherry.  Two DAPI sets are available allowing users to choose between a narrowband excitation filter to minimize photobleaching and phototoxicity to the sample, and a wide exciter for maximum signal in very low-concentration samples.  Users of current DAPI, GFP or mCherry BrightLine filter sets may quickly upgrade to long-pass sets by just dropping in the new long-pass emission filters purchased separately.  All three emitters are guaranteed to offer > 93% average transmission over the passband and extremely steep edges that are perfect for capturing fluorescence as close to the excitation band as possible for maximum efficiency.

Semrock’s industry-leading and proprietary hard-coated filter manufacturing process ensures permanent performance with “no burn-out” even under the most intense illumination conditions.  Semrock filters are available from stock for same-day shipment with a 30-day no-hassle return policy and are covered under the company’s five-year warranty. Custom-sized filters are available in under a week.

Posted by Lee Mather

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