PicoQuant FLIM upgrade kit for Olympus two-photon confocal microscope

Click to Enlarge

A FLIM image of a cell with a GFP/RFP tandem construct. The red stripe is caused by acceptor photobleaching in this area that prevents an energy transfer-induced decrease in the fluorescence lifetime of the GFP.

The Fluorescence Lifetime (FLIM) upgrade kit from PicoQuant (Berlin, Germany) is now also available for Olympus' FluoView FV1000MPE two-photon confocal microscope with non-descanned detection (NDD). The upgrade kit extends the capabilities of the microscope towards time-resolved FLIM measurements with a temporal resolution down to picoseconds. The kit includes a small modification to the NDD unit of the Olympus MPE system to enable switching between NDD imaging and FLIM via a change of cables. Up to four internal NDD detectors can be used for FLIM.

-----

PRESS RELEASE

FLIM upgrade kit for Olympus FV1000MPE with Non-descanned Detection (NDD)

The Fluorescence Lifetime (FLIM) upgrade kit from PicoQuant has been further improved
and is now also available for the new two photon confocal microscope from Olympus, the FluoView FV1000MPE with non-descanned detection (NDD). The upgrade kit extends the capabilities of the microscope towards time-resolved FLIM measurements with a temporal resolution down to picoseconds and allows users to perform various analysis on this time scale.

The kit includes a small modification to the NDD unit of the Olympus MPE system, which enables the user to easily switch between standard NDD imaging and FLIM via a simple change of cables. Up to 4 internal NDD detectors can be used for FLIM. The kit uses the two photon laser source of the FV1000MPE system as excitation source and is completed by Time-Correlated Single Photon Counting (TCSPC) electronics and a special data acquisition and analysis software for FLIM and FLIM-FRET.

Due to the usage of many components already present, the upgrade kit is an economical way to extend the capabilities of the FV1000MPE towards FLIM measurements. NDD FLIM is extremely suited for deep tissue FLIM, where descanned detection becomes very insensitive. Still, the FV1000MPE can also be combined with sensitive descanned detection by additionally attaching Single Photon Avalanche Diodes (SPADs) with a extremely high quantum efficiency. This would make additional measurement modes like Fluorescence Correlation Spectroscopy (FCS) possible.

 

Get All the BioOptics World News Delivered to Your Inbox

Subscribe to BioOptics World Magazine or email newsletter today at no cost and receive the latest news and information.

 Subscribe Now
Related Articles

New bioimaging technique offers clear view of nervous system

Scientists at Ludwig-Maximilians University have developed a technique for turning the body of a deceased rodent entirely transparent, revealing the central nervous system in unprecedented clarity....

Fluorescent jellyfish proteins light up unconventional laser

Safer lasers to map your cells could soon be in the offing -- all thanks to the humble jellyfish. Conventional lasers, like the pointer you might use to entertain your cat, produce light by emittin...

Fluorescence microscopy helps provide new insight into how cancer cells metastasize

By using fluorescence microscopy, scientists have discovered an alternate theory on how some cancer cells metastasize.

In vivo imaging method visualizes bone-resorbing cell function in real time

In vivo imaging can visualize sites where osteoclasts (bone-resorbing cells) were in the process of resorbing bone.

BLOGS

Neuro15 exhibitors meet exacting demands: Part 2

Increasingly, neuroscientists are working with researchers in disciplines such as chemistry and p...

Why be free?

A successful career contributed to keeping OpticalRayTracer—an optical design software program—fr...

LASER Munich 2015 is bio-bent

LASER World of Photonics 2015 included the European Conferences on Biomedical Optics among its si...

White Papers

Understanding Optical Filters

Optical filters can be used to attenuate or enhance an image, transmit or reflect specific wavele...

How can I find the right digital camera for my microscopy application?

Nowadays, image processing is found in a wide range of optical microscopy applications. Examples ...

CONNECT WITH US

            

Twitter- BioOptics World

Copyright © 2007-2016. PennWell Corporation, Tulsa, OK. All Rights Reserved.PRIVACY POLICY | TERMS AND CONDITIONS