Hi-res TIRF microscopy system by Carl Zeiss

With Laser TIRF 3, Carl Zeiss infuses its imaging system for Total Internal Reflection Fluorescence microscopy with improved quality. The TIRF system is suitable for reproducible microscopic examinations of near-cell membrane dynamic processes and interactions of single molecules in cell-free systems with high time resolution. Laser TIRF 3 can be combined with other procedures and techniques, such as atomic force microscopy (AFM). It therefore meets user requirements in the biosciences, biophysics, biochemistry and molecular biology even more effectively.

The technical features of TIRF 3 enable a wide variety of experimental possibilities. The TIRF slider is available in two versions, i.e. with manual and motorized angle setting. The new laser module offers a more flexible choice of dyes. It can be equipped with up to four different solid-state lasers (405, 488 nm or Ar laser, 532 or 561, 635 nm). The laser module is AOTF-controlled and completely operable via the AxioVision software.

Additional features include high time resolution, reproducible angle settings and therefore reproducible depths of penetration. AOTF control and angle setting are integrated into the "fast image acquisition" concept of the AxioVision software from Carl Zeiss, resulting in a markedly higher time resolution of the images. The motorized angle setting in the TIRF slider permits a given illumination angle to be set reproducibly and considerably faster than with standard systems. Together with the corrected beam path and special filter sets, the apochromatically corrected optics of the TIRF slider guarantee maximum image quality. Combining Laser TIRF 3 with incubation accessories and the Definite Focus module from Carl Zeiss enables users to reliably obtain quantitative data from their experiments even over longer periods of time. This makes Laser TIRF 3 a genuine analysis tool.

More information:
Laser TIRF 3

Get All the BioOptics World News Delivered to Your Inbox

Subscribe to BioOptics World Magazine or email newsletter today at no cost and receive the latest news and information.

 Subscribe Now
Related Articles

Four-lens light-sheet microscope delivers whole-embryo images in real time

A team of researchers from the Max Planck Institute and Technical University (both in Dresden, Germany) has created the first microscope that processes image data in real time and provides the rese...

Biophotonic Solutions closes $1M funding round

Automated laser pulse compression technology developer Biophotonic Solutions Inc. (BSI) has closed a $1 million Series A funding round led by the Michigan Angel Fund.

Microscopy helps discover potential new drug target for cystic fibrosis

An international team of scientists, using automated microscopy and genetics, have discovered a promising potential drug target for cystic fibrosis.

(SLIDE SHOW) Photonics on the brain

Photonics are helping to measure, manipulate, and follow molecular events in living neurons, which could lead to treatments, cures, and possibly even preventions of brain disorders and diseases.


Neuro15 exhibitors meet exacting demands: Part 2

Increasingly, neuroscientists are working with researchers in disciplines such as chemistry and p...

Why be free?

A successful career contributed to keeping OpticalRayTracer—an optical design software program—fr...

LASER Munich 2015 is bio-bent

LASER World of Photonics 2015 included the European Conferences on Biomedical Optics among its si...

White Papers

Understanding Optical Filters

Optical filters can be used to attenuate or enhance an image, transmit or reflect specific wavele...

How can I find the right digital camera for my microscopy application?

Nowadays, image processing is found in a wide range of optical microscopy applications. Examples ...



Twitter- BioOptics World