Looking at thought

HEIDELBERG, GERMANY -- An international team of scientists, headed by Mazahir Hasan of the Max Planck Institute for Medical Research, has succeeded in optically detecting individual action potentials in the brains of living animals. The scientists introduced fluorescent indicator proteins into the brain cells of mice via viral gene vectors: Illumination of the proteins indicates both when and which neurons are communicating with each other.

This new method enables the observation of brain activity over a period of many months and provides new ways of identifying, for example, the early onset of dysfunction in neurological disorders such as Alzheimer's and Parkinson's. The fluorescent proteins could also provide scientists with information about the ways in which normal aging processes affect nerve cell communication.

Neural communication system
A nerve cell is a major hub for the exchange of valuable information. Our sense organs perceive our environment through various receptors, and pass the stimuli to neurons -- which collect, process, and transfer the information to specific brain centers at these hubs. The nerve cell uses a special means of transport for this purpose: the action potential that codes the information, thus enabling communication between the nerve cells.

An action potential of this kind is an electrical excitation and arises when our nerve cells receive the information via a stimulus. Shortly before the nerve cell forwards the information via the stimulus, calcium ions pour into the nerve cell, acting as the starting gun for the flow of data from one neuron to the next.

Action potential has typically been measured and rendered visible using microelectrodes. But this method enables monitoring of only a limited number of cells engaged in communication. Moreover, it cannot record communication in a clearly identifiable way over a longer period or in freely moving animals.

Yellow and blue fluorescent proteins
As part of an intensive international cooperation project, Mazahir Hasan has made nerve cells, which release a single action potential, optically visible in mice. This means that the communication of entire groups of neurons can be observed over an extended period of time. Mazahir Hasan also attracted attention in 2004 when he demonstrated for the first time that fluorescent proteins are suitable for making visible activity in the brains of mice (Hasan et al., 2004 PLoS Biology 2:e163).

For this new development, Hasan used a sensor protein called D3cpv, generated by Amy Palmer at the Roger Tsien Laboratory of the University of California in San Diego, as a complex of numerous interconnected protein subunits. Two of these subunits react to the binding of calcium ions to the complex: the yellow-fluorescent protein (YFP) lights up and the illuminating power of cyan-fluorescent protein (CFP) declines -- a coincidence that proved crucial to the success of the study.

The Max Planck scientists introduced the corresponding genetic material -- that is the construction manual for this protein complex -- into the genetic material of viruses. Hasan and his team then used these viruses as a genetic "ferry" for introducing the genetic material into the brains of mice. The protein complex was actually produced in the nerve cells of the "infected" mice and functions there as an calcium indicator: if the calcium level within a cell increases -- which is the case with every action potential -- the D3cpv changes form when it binds to calcium. As a result, the two fluorescent proteins, CFP and YFP, move closer to each other and the transmission of energy between the CFP and YFP changes.

"To observe this change, we use a two-photon microscope developed by Winfried Denk," explains Hasan. Each individual action potential that arises due to a stimulus makes itself directly perceivable in the brain through yellow illumination and the simultaneous reduction in the emission of blue light. The two-photon microscope pinpoints the coincidence between the two fluorescent signals very accurately and clearly reveals which nerve cells are communicating and exchanging information with each other and when.

Damian Wallace and Jason Kerr from the Max Planck Institute for Biological Cybernetics in Tübingen were able to confirm this finding: targeted electrical recordings of neuronal activity after the triggering of stimulus showed that the color change actually coincides with the firing of the action potentials. Hasan's method sheds light on which nerve cells will talk to each other and in which time period. However, it is only applicable if the neurons fire action potentials with a frequency of less than one hertz.

Insight into complex thought processes
The researchers were thus able to demonstrate -- apparently for the first time -- that genetic calcium indicators provide optical proof of the perceptions of the sensory system in higher organisms. "With this method we can understand, in greater detail, how the human brain regulates complex thought processes and, for example, how it transforms the numerous sensory impressions into long-term memories," says Hasan. Developments resulting from the aging of the nerve cells can also be understood better as a result -- "as we now have a way of observing the neurons over longer periods of time," concludes Hasan. Moreover, the sensor proteins could prove very useful in helping researchers to reach a better understanding at the cellular level of neurological diseases including Alzheimer's, Parkinson's, and Huntington's chorea.

More information:
Damian J Wallace, Stephan Meyer zum Alten Borgloh, Simone Astori, Ying Yang, Melanie Bausen, Sebastian Kügler, Amy E Palmer, Roger Y Tsien, Rolf Sprengel, Jason N D Kerr, Winfried Denk & Mazahir T Hasan
Single-spike detection in vitro and in vivo with a genetic Ca2+ sensor
Nature Methods, Vol.5 No. 9, September 2008, 797

Mazahir T Hasan, Rainer W Friedrich, Thomas Euler, Matthew E Larkum, Günter Giese, Matthias Both, Jens Duebel, Jack Waters, Hermann Bujard, Oliver Griesbeck, Roger Y Tsien, Takeharu Nagai, Atsushi Miyawaki, Winfried Denk
Functional fluorescent Ca2+ indicator proteins in transgenic mice under TET control
PLOS Biology, Vol.2 No.6, June 2004, e163

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